Open AccessImproved Sensitivity of Intramolecular Strand Displacement Based on Localization of Probes
Author(s) -
Seung Won Shin,
So Yeon Ahn,
Yong Taik Lim,
Soong Ho Um
Publication year - 2019
Publication title -
analytical chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.117
H-Index - 332
eISSN - 1520-6882
pISSN - 0003-2700
DOI - 10.1021/acs.analchem.9b03173
Subject(s) - chemistry , intramolecular force , sensitivity (control systems) , nanostructure , biosensor , nanotechnology , intermolecular force , biological system , dna , flexibility (engineering) , biophysics , molecule , stereochemistry , biochemistry , electronic engineering , materials science , statistics , organic chemistry , engineering , biology , mathematics
Effective intermolecular interaction is required between probe and target molecules for successful detection of biomarkers. Here, we demonstrate that localization of probes on DNA nanostructures improves detection sensitivity and reaction rate. The structural flexibility of DNA nanostructures enabled frequent intramolecular interactions among the localized probes. The Smoluchowski coagulation method and the coarse-grained molecular dynamic software oxDNA were used for theoretical estimation of inter- and intramolecular behaviors of the DNA nanostructures as well as adequate experiments verifying the improvements in sensitivity with probe localization. Remarkably, the probe-localized DNA nanostructure had an increased sensitivity up to 274 times higher than that of the same probes without localization. We believe this achievement represents a wide applicability as a potential design strategy for robust, reliable, and sensitive biosensors.
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