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PDMS is biocompatible, economically viable, transparent, and facile to handle and thus is suitable for fluorescent microscopy and biological research. However, there has been no report about polysiloxane-based fluorescent probes applied in bioimaging. In this report, a two-photon polysiloxane-based reversible luminescent probe (P1) was fabricated for the first time. P1 is a powerful tool for detecting the ClO - /GSH cycle in situ both in live cells and in zebrafish. This work demonstrates the potential of polysiloxane-based fluorescent probes for versatile in vivo or in vitro applications in the future.

analytical chemistry

Binding Reaction Sites to Polysiloxanes: Unique Fluorescent Probe for Reversible Detection of ClO<sup>–</sup>/GSH Pair and the in Situ Imaging in Live Cells and Zebrafish

Binding Reaction Sites to Polysiloxanes: Unique Fluorescent Probe for Reversible Detection of ClO–/GSH Pair and the in Situ Imaging in Live Cells and Zebrafish

Binding Reaction Sites to Polysiloxanes: Unique Fluorescent Probe for Reversible Detection of ClO^–/GSH Pair and the in Situ Imaging in Live Cells and Zebrafish