Legumain-Specific Near-Infrared Fluorescence “Turn On” for Tumor-Targeted Imaging | Zendy

Yue Zhao | Zendy; Zijuan Hai | Zendy; Hongyong Wang | Zendy; Lanhong Su | Zendy; Gaolin Liang | Zendy

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Legumain-Specific Near-Infrared Fluorescence “Turn On” for Tumor-Targeted Imaging

Author(s) -

Yue Zhao,

Zijuan Hai,

Hongyong Wang,

Lanhong Su,

Gaolin Liang

Publication year - 2018

Publication title -

analytical chemistry

Language(s) - Uncategorized

Resource type - Journals

SCImago Journal Rank - 2.117

H-Index - 332

eISSN - 1520-6882

pISSN - 0003-2700

DOI - 10.1021/acs.analchem.8b02704

Subject(s) - nanoprobe , chemistry , fluorescence , near infrared spectroscopy , in vivo , nanotechnology , biophysics , fluorescence lifetime imaging microscopy , nanoparticle , materials science , neuroscience , biology , physics , microbiology and biotechnology , quantum mechanics

Legumain is one of the cysteine proteases which can serve as an essential indicator for cancer diagnosis. Near-infrared (NIR) nanoprobes with fluorescence "Turn On" property are advantageous in cancer diagnosis. However, to the best of our knowledge, using a completely organic NIR nanoprobe to image legumain activity either in vitro or in vivo has not been reported. Herein, employing a CBT-Cys click condensation reaction, we used a rationally designed NIR probe Cys(StBu)-Ala-Ala-Asn-Lys(Cy5.5)-CBT (1) to synthesize its nanoprobes 1-NPs with self-quenched fluorescence. Cell and animal experiments indicated that our nanoprobes were able to specifically image legumain activity in living cells and tumors with a NIR fluorescence "Turn On" manner. We envision that the nanoprobes could be applied for the diagnosis of legumain-related diseases in the near future.

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