Quantitation of Intact Proteins in Human Plasma Using Top-Down Parallel Reaction Monitoring-MS | Zendy

Yuchao Chen | Zendy; Pan Mao | Zendy; Daojing Wang | Zendy

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Quantitation of Intact Proteins in Human Plasma Using Top-Down Parallel Reaction Monitoring-MS

Author(s) -

Yuchao Chen,

Pan Mao,

Daojing Wang

Publication year - 2018

Publication title -

analytical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.117

H-Index - 332

eISSN - 1520-6882

pISSN - 0003-2700

DOI - 10.1021/acs.analchem.8b02699

Subject(s) - chemistry , human plasma , chromatography , mass spectrometry , microfluidic chip , microfluidics , selected reaction monitoring , liquid chromatography–mass spectrometry , tandem mass spectrometry , nanotechnology , materials science

Direct quantitation of proteins in complex biological matrices by mass spectrometry remains a challenge. Here, we describe a novel top-down parallel reaction monitoring-mass spectrometry (PRM-MS) assay, enabled by microflow LC-nanospray MS using a silicon microfluidic LC-MS chip. We demonstrated direct analysis of intact proteins such as somatropin in human plasma, achieving sensitivity (0.1-1.0 fmole) and speed (1-5 min) on par with enzyme-linked immunosorbent assay (ELISA).

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