Endogenous Alpha-Synuclein Protein Analysis from Human Brain Tissues Using Single-Molecule Pull-Down Assay | Zendy

Goun Je | Zendy; Benjamin Croop | Zendy; Sambuddha Basu | Zendy; Jialei Tang | Zendy; Kyu Young Han | Zendy; Yoon-Seong Kim | Zendy

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Endogenous Alpha-Synuclein Protein Analysis from Human Brain Tissues Using Single-Molecule Pull-Down Assay

Author(s) -

Goun Je,

Benjamin Croop,

Sambuddha Basu,

Jialei Tang,

Kyu Young Han,

Yoon-Seong Kim

Publication year - 2017

Publication title -

analytical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.117

H-Index - 332

eISSN - 1520-6882

pISSN - 0003-2700

DOI - 10.1021/acs.analchem.7b04335

Subject(s) - chemistry , alpha synuclein , endogeny , human brain , in vivo , parkinson's disease , pathogenesis , disease , computational biology , biochemistry , neuroscience , pathology , biology , genetics , medicine

Alpha-synuclein (α-SYN) is a central molecule in Parkinson's disease pathogenesis. Despite several studies, the molecular nature of endogenous α-SYN especially in human brain samples is still not well understood due to the lack of reliable methods and the limited amount of biospecimens. Here, we introduce α-SYN single-molecule pull-down (α-SYN SiMPull) assay combined with in vivo protein crosslinking to count individual α-SYN protein and assess its native oligomerization states from biological samples including human postmortem brains. This powerful single-molecule assay can be highly useful in diagnostic applications using various specimens for neurodegenerative diseases including Alzheimer's disease and Parkinson's disease.

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