Quantitative Analysis of Ligand Induced Heterodimerization of Two Distinct Receptors | Zendy

Chang Lu | Zendy; ZhiXin Wang | Zendy

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Quantitative Analysis of Ligand Induced Heterodimerization of Two Distinct Receptors

Author(s) -

Chang Lu,

ZhiXin Wang

Publication year - 2017

Publication title -

analytical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.117

H-Index - 332

eISSN - 1520-6882

pISSN - 0003-2700

DOI - 10.1021/acs.analchem.7b01274

Subject(s) - chemistry , förster resonance energy transfer , receptor , fkbp , ligand (biochemistry) , biophysics , biological system , monomer , computational biology , fluorescence , biochemistry , physics , polymer , organic chemistry , quantum mechanics , biology

The induced dimerization of two distinct receptors through a heterobifunctional inducer is prevalent among all levels of cellular signaling processes, yet its complexity poses difficulty for systematic quantitative analysis. This paper first shows how to calculate the amount of any possible complex or monomer of heteroligand and two receptors present at equilibrium. The theory is subsequently applied to the determination of three independent equilibrium parameters involved in the rapamycin induced FKBP and FRB dimerization, in which all parameters were simultaneously estimated using one set of fluorescence resonance energy transfer (FRET) experiments. A MATLAB script is provided for parametric fitting.

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