Quantitative Nonaqueous Capillary Electrophoresis–Mass Spectrometry Method for Determining Active Ingredients in Plant Extracts | Zendy

Jianhui Cheng | Zendy; Lingyu Wang | Zendy; Liu Wei-fen | Zendy; David D. Y. Chen | Zendy

Open Access

Quantitative Nonaqueous Capillary Electrophoresis–Mass Spectrometry Method for Determining Active Ingredients in Plant Extracts

Author(s) -

Jianhui Cheng,

Lingyu Wang,

Liu Wei-fen,

David D. Y. Chen

Publication year - 2017

Publication title -

analytical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.117

H-Index - 332

eISSN - 1520-6882

pISSN - 0003-2700

DOI - 10.1021/acs.analchem.6b04944

Subject(s) - chemistry , chromatography , capillary electrophoresis , mass spectrometry , capillary electrophoresis–mass spectrometry , reproducibility , emodin , electrolyte , analytical chemistry (journal) , electrospray ionization , electrode

Nonaqueous capillary electrophoresis (NACE) is very well suited for online coupling with mass spectrometry due to the relatively high volatility and low surface tension of most organic solvents. Here we present a quantitative NACE-ESI-MS/MS method for separating and determining physcion, chrysophanol, and aloe-emodin in rhubarb. Dantron was used as an internal standard to ensure accuracy and reproducibility in quantitative analyses. Parameters including the pH, background electrolyte (BGE) composition, flow-through microvial chemical modifier solution composition, and modifier solution flow rate were carefully optimized. The developed method was validated by assessing its precision, LODs, and linear range. The contents of physcion, chrysophanol, and aloe-emodin in rhubarb were determined to be 0.22%, 1.0%, and 0.17%, respectively.

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