Open AccessTandem Mass Spectrometry in Combination with Product Ion Mobility for the Identification of Phospholipids
Author(s) -
Karin A. Zemski Berry,
Robert M. Barkley,
Joseph J. Berry,
Joseph A. Hankin,
Emmy Hoyes,
Jeffery M. Brown,
Robert C. Murphy
Publication year - 2016
Publication title -
analytical chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.117
H-Index - 332
eISSN - 1520-6882
pISSN - 0003-2700
DOI - 10.1021/acs.analchem.6b04047
Subject(s) - chemistry , ion mobility spectrometry , ion , tandem mass spectrometry , mass spectrometry , lipidomics , top down proteomics , analytical chemistry (journal) , tandem , chromatography , selected reaction monitoring , biochemistry , organic chemistry , materials science , composite material
Concerted tandem and traveling wave ion mobility mass spectrometry (CTS analysis) is a unique method that results in a four-dimensional data set including nominal precursor ion mass, product ion mobility, accurate mass of product ion, and ion abundance. This nontargeted lipidomics CTS approach was applied in both positive- and negative-ion mode to phospholipids present in human serum, and the data set was used to evaluate the value of product ion mobility in identifying lipids in a complex mixture. It was determined that the combination of diagnostic product ions and unique collisional cross-section values of product ions is a powerful tool in the structural identification of lipids in a complex biological sample.