Construction of Epidermal Growth Factor Receptor Peptide Magnetic Nanovesicles with Lipid Bilayers for Enhanced Capture of Liver Cancer Circulating Tumor Cells | Zendy

Jian Ding | Zendy; Kai Wang | Zendy; Wenjie Tang | Zendy; Dan Li | Zendy; Youzhen Wei | Zendy; Ying Lü | Zendy; ZongHai Li | Zendy; Xiaofei Liang | Zendy

Open Access

Construction of Epidermal Growth Factor Receptor Peptide Magnetic Nanovesicles with Lipid Bilayers for Enhanced Capture of Liver Cancer Circulating Tumor Cells

Author(s) -

Jian Ding,

Kai Wang,

Wenjie Tang,

Dan Li,

Youzhen Wei,

Ying Lü,

ZongHai Li,

Xiaofei Liang

Publication year - 2016

Publication title -

analytical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.117

H-Index - 332

eISSN - 1520-6882

pISSN - 0003-2700

DOI - 10.1021/acs.analchem.6b01443

Subject(s) - chemistry , fluorescein isothiocyanate , epidermal growth factor receptor , peptide , circulating tumor cell , epidermal growth factor , cancer cell , vesicle , epithelial cell adhesion molecule , biophysics , cancer , liver cancer , receptor , cancer research , cell , biochemistry , fluorescence , membrane , hepatocellular carcinoma , metastasis , medicine , physics , quantum mechanics , biology

Highly effective targeted tumor recognition via vectors is crucial for cancer detection. In contrast to antibodies and proteins, peptides are direct targeting ligands with a low molecular weight. In the present study, a peptide magnetic nanovector platform containing a lipid bilayer was designed using a peptide amphiphile (PA) as a skeleton material in a controlled manner without surface modification. Fluorescein isothiocyanate-labeled epidermal growth factor receptor (EGFR) peptide nanoparticles (NPs) could specifically bind to EGFR-positive liver tumor cells. EGFR peptide magnetic vesicles (EPMVs) could efficiently recognize and separate hepatoma carcinoma cells from cell solutions and treated blood samples (ratio of magnetic EPMVs versus anti-EpCAM NPs: 3.5 ± 0.29). Analysis of the circulating tumor cell (CTC) count in blood samples from 32 patients with liver cancer showed that EPMVs could be effectively applied for CTC capture. Thus, this nanoscale, targeted cargo-packaging technology may be useful for designing cancer diagnostic systems.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research