Dark Field Transmission Electron Microscopy as a Tool for Identifying Inorganic Nanoparticles in Biological Matrices | Zendy

Nathan D. Klein | Zendy; Katie R. Hurley | Zendy; Z. Vivian Feng | Zendy; Christy L. Haynes | Zendy

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Dark Field Transmission Electron Microscopy as a Tool for Identifying Inorganic Nanoparticles in Biological Matrices

Author(s) -

Nathan D. Klein,

Katie R. Hurley,

Z. Vivian Feng,

Christy L. Haynes

Publication year - 2015

Publication title -

analytical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.117

H-Index - 332

eISSN - 1520-6882

pISSN - 0003-2700

DOI - 10.1021/acs.analchem.5b00124

Subject(s) - dark field microscopy , nanomaterials , nanotechnology , chemistry , nanoparticle , transmission electron microscopy , field (mathematics) , nanomedicine , microscopy , optics , materials science , physics , mathematics , pure mathematics

Dark field transmission electron microscopy has been applied herein to visualize the interactions of inorganic nanomaterials with biological systems. This new application of a known technique addresses a deficiency in status quo visualization techniques. High resolution and low noise images can be acquired to locate and identify crystalline nanoparticles in complex biological matrices. Moreover, through the composition of multiple images taken at different angular beam tilts, it is possible to image a majority of nanoparticles present at a site in dark field mode. This facilitates clarity regarding the internalization of nanomaterials in cellular systems. In addition, comparing dark field images recorded at different angular tilts yields insight into the character of nanoparticle faceting.

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