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Development and Validation of a New Robust Detection Method for Low-Content DNA Using ΔΔCq-Based Real-Time PCR with Optimized Standard Plasmids as a Control Sample
Author(s) -
Keisuke Soga,
Kôsuke Nakamura,
Tomohiro Egi,
Jumpei Narushima,
Satoko Yoshiba,
Masahiro Kishine,
Junichi Mano,
Kazumi Kitta,
Reona Takabatake,
Norihito Shibata,
Kazunari Kondo
Publication year - 2022
Publication title -
analytical chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.117
H-Index - 332
eISSN - 1520-6882
pISSN - 0003-2700
DOI - 10.1021/acs.analchem.2c03680
Subject(s) - chemistry , genomic dna , detection limit , dna , real time polymerase chain reaction , polymerase chain reaction , plasmid , chromatography , microbiology and biotechnology , gene , biology , biochemistry

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