Mitokyne: A Ratiometric Raman Probe for Mitochondrial pH | Zendy

Liam T. Wilson | Zendy; William J. Tipping | Zendy; Corinna Wetherill | Zendy; Z.A. Henley | Zendy; Karen Faulds | Zendy; Duncan Graham | Zendy; Simon P. Mackay | Zendy; Nicholas C. O. Tomkinson | Zendy

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Mitokyne: A Ratiometric Raman Probe for Mitochondrial pH

Author(s) -

Liam T. Wilson,

William J. Tipping,

Corinna Wetherill,

Z.A. Henley,

Karen Faulds,

Duncan Graham,

Simon P. Mackay,

Nicholas C. O. Tomkinson

Publication year - 2021

Publication title -

analytical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.117

H-Index - 332

eISSN - 1520-6882

pISSN - 0003-2700

DOI - 10.1021/acs.analchem.1c03075

Subject(s) - chemistry , mitophagy , organelle , biophysics , raman spectroscopy , mitochondrion , electron transport chain , atp synthase , raman scattering , function (biology) , biochemistry , microbiology and biotechnology , nanotechnology , enzyme , physics , autophagy , optics , materials science , biology , apoptosis

Mitochondrial pH (pH mito ) is intimately related to mitochondrial function, and aberrant values for pH mito are linked to several disease states. We report the design, synthesis, and application of mitokyne 1 -the first small molecule pH mito sensor for stimulated Raman scattering (SRS) microscopy. This ratiometric probe can determine subtle changes in pH mito in response to external stimuli and the inhibition of both the electron transport chain and ATP synthase with small molecule inhibitors. In addition, 1 was also used to monitor mitochondrial dynamics in a time-resolved manner with subcellular spatial resolution during mitophagy providing a powerful tool for dissecting the molecular and cell biology of this critical organelle.

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