Rapid Identification of SARS-CoV-2 Variants of Concern Using a Portable peakPCR Platform | Zendy

Philippe Bechtold | Zendy; Philipp Wagner | Zendy; Salome Hosch | Zendy; Denise Siegrist | Zendy; Amalia Ruiz-Serrano | Zendy; Michele Gregorini | Zendy; Maxmillian Mpina | Zendy; Florentino Abaga Ondó | Zendy; Justino Obama | Zendy; Mitoha Ondo’o Ayekaba | Zendy; Olivier Engler | Zendy; Wendelin J. Stark | Zendy; Claudia Daubenberger | Zendy; Tobias Schindler | Zendy

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Rapid Identification of SARS-CoV-2 Variants of Concern Using a Portable peakPCR Platform

Author(s) -

Philippe Bechtold,

Philipp Wagner,

Salome Hosch,

Denise Siegrist,

Amalia Ruiz-Serrano,

Michele Gregorini,

Maxmillian Mpina,

Florentino Abaga Ondó,

Justino Obama,

Mitoha Ondo’o Ayekaba,

Olivier Engler,

Wendelin J. Stark,

Claudia Daubenberger,

Tobias Schindler

Publication year - 2021

Publication title -

analytical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.117

H-Index - 332

eISSN - 1520-6882

pISSN - 0003-2700

DOI - 10.1021/acs.analchem.1c02368

Subject(s) - covid-19 , identification (biology) , computational biology , single nucleotide polymorphism , virology , gene , biology , genotype , genetics , medicine , pathology , botany , disease , outbreak , infectious disease (medical specialty)

The need for tools that facilitate rapid detection and continuous monitoring of SARS-CoV-2 variants of concern (VOCs) is greater than ever, as these variants are more transmissible and therefore increase the pressure of COVID-19 on healthcare systems. To address this demand, we aimed at developing and evaluating a robust and fast diagnostic approach for the identification of SARS-CoV-2 VOC-associated spike gene mutations. Our diagnostic assays detect the E484K and N501Y single-nucleotide polymorphisms (SNPs) as well as a spike gene deletion (HV69/70) and can be run on standard laboratory equipment or on the portable rapid diagnostic technology platform peak PCR. The assays achieved excellent diagnostic performance when tested with RNA extracted from culture-derived SARS-CoV-2 VOC lineages and clinical samples collected in Equatorial Guinea, Central-West Africa. Simplicity of usage and the relatively low cost are advantages that make our approach well suitable for decentralized and rapid testing, especially in resource-limited settings.

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