Visualizing Cholesterol in the Brain by On-Tissue Derivatization and Quantitative Mass Spectrometry Imaging | Zendy

Roberto Angelini | Zendy; Eylan Yutuc | Zendy; Mark F. Wyatt | Zendy; Jillian Newton | Zendy; Fowzi Adam Yusuf | Zendy; Lauren Griffiths | Zendy; Benjamin Cooze | Zendy; Dana El Assad | Zendy; Gilles Frache | Zendy; Wei Rao | Zendy; Luke B. Allen | Zendy; Z̆eljka Korade | Zendy; Thu T. A. Nguyen | Zendy; Rathnayake A. C. Rathnayake | Zendy; Stephanie M. Cologna | Zendy; Owain W. Howell | Zendy; Malcolm R. Clench | Zendy; Yuqin Wang | Zendy; William J. Griffiths | Zendy

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Visualizing Cholesterol in the Brain by On-Tissue Derivatization and Quantitative Mass Spectrometry Imaging

Author(s) -

Roberto Angelini,

Eylan Yutuc,

Mark F. Wyatt,

Jillian Newton,

Fowzi Adam Yusuf,

Lauren Griffiths,

Benjamin Cooze,

Dana El Assad,

Gilles Frache,

Wei Rao,

Luke B. Allen,

Z̆eljka Korade,

Thu T. A. Nguyen,

Rathnayake A. C. Rathnayake,

Stephanie M. Cologna,

Owain W. Howell,

Malcolm R. Clench,

Yuqin Wang,

William J. Griffiths

Publication year - 2021

Publication title -

analytical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.117

H-Index - 332

eISSN - 1520-6882

pISSN - 0003-2700

DOI - 10.1021/acs.analchem.0c05399

Subject(s) - mass spectrometry imaging , chemistry , cholesterol , derivatization , mass spectrometry , sterol , corpus callosum , pons , brain tissue , biochemistry , medicine , chromatography , neuroscience , psychology

Despite being a critical molecule in the brain, mass spectrometry imaging (MSI) of cholesterol has been under-reported compared to other lipids due to the difficulty in ionizing the sterol molecule. In the present work, we have employed an on-tissue enzyme-assisted derivatization strategy to improve detection of cholesterol in brain tissue sections. We report distribution and levels of cholesterol across specific structures of the mouse brain, in a model of Niemann-Pick type C1 disease, and during brain development. MSI revealed that in the adult mouse, cholesterol is the highest in the pons and medulla and how its distribution changes during development. Cholesterol was significantly reduced in the corpus callosum and other brain regions in the Npc1 null mouse, confirming hypomyelination at the molecular level. Our study demonstrates the potential of MSI to the study of sterols in neuroscience.

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