Open AccessRapid One-Pot Detection of SARS-CoV-2 Based on a Lateral Flow Assay in Clinical Samples
Author(s) -
Chao Zhang,
Tingting Zheng,
Hua Wang,
Wei Chen,
Xiaoye Huang,
Jianqi Liang,
Liping Qiu,
Da Han,
Weihong Tan
Publication year - 2021
Publication title -
analytical chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.117
H-Index - 332
eISSN - 1520-6882
pISSN - 0003-2700
DOI - 10.1021/acs.analchem.0c05059
Subject(s) - loop mediated isothermal amplification , point of care testing , chemistry , covid-19 , point of care , pathogen , nucleic acid , diagnostic test , nucleic acid detection , infectious disease (medical specialty) , virology , chromatography , microbiology and biotechnology , disease , pathology , immunology , veterinary medicine , biology , medicine , dna , biochemistry
Rapid tests for pathogen identification and spread assessment are critical for infectious disease control and prevention. The control of viral outbreaks requires a nucleic acid diagnostic test that is sensitive and simple and delivers fast and reliable results. Here, we report a one-pot direct reverse transcript loop-mediated isothermal amplification (RT-LAMP) assay of SARS-CoV-2 based on a lateral flow assay in clinical samples. The entire contiguous sample-to-answer workflow takes less than 40 min from a clinical swab sample to a diagnostic result without professional instruments and technicians. The assay achieved an accuracy of 100% in 12 synthetic and 12 clinical samples compared to the data from PCR-based assays. We anticipate that our method will provide a universal platform for rapid and point-of-care detection of emerging infectious diseases.
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