Open AccessSub-100 μm Spatial Resolution Ambient Mass Spectrometry Imaging of Rodent Brain with Laser Ablation Atmospheric Pressure Photoionization (LAAPPI) and Laser Ablation Electrospray Ionization (LAESI)
Author(s) -
Juha-Pekka Hieta,
Jaakko Kopra,
Heikki Räikkönen,
Tiina J. Kauppila,
Risto Kostiainen
Publication year - 2020
Publication title -
analytical chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.117
H-Index - 332
eISSN - 1520-6882
pISSN - 0003-2700
DOI - 10.1021/acs.analchem.0c01597
Subject(s) - chemistry , photoionization , mass spectrometry , laser ablation , laser , resolution (logic) , ionization , ablation , analytical chemistry (journal) , electrospray ionization , atmospheric pressure , mass spectrometry imaging , ion , optics , chromatography , physics , oceanography , organic chemistry , engineering , artificial intelligence , geology , computer science , aerospace engineering
In this study, we applied a new IR laser-beam-focusing technique to enable sub-100 μm spatial resolution in laser ablation atmospheric pressure photoionization (LAAPPI) and laser ablation electrospray ionization (LAESI) mass spectrometry imaging (MSI). After optimization of operational parameters, both LAAPPI- and LAESI-MSI with a spatial resolution of 70 μm produced high-quality MS images, which allowed accurate localization of metabolites and lipids in the mouse and rat brain. Negative and positive ion LAAPPI- and LAESI-MS detected many of the same metabolites and lipids in the brain. Many compounds were also detected either by LAAPPI- or LAESI-MS, indicating that LAAPPI and LAESI are more complementary than alternative methods.
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