DNA Interaction with Palladium Chelates of Biogenic Polyamines Using Atomic Force Microscopy and Voltammetric Characterization | Zendy

Oana Corduneanu | Zendy; AnaMaria ChiorceaPaquim | Zendy; Victor C. Diculescu | Zendy; Sónia M. Fiuza | Zendy; M. Paula M. Marques | Zendy; Ana Maria OliveiraBrett | Zendy

Open Access

DNA Interaction with Palladium Chelates of Biogenic Polyamines Using Atomic Force Microscopy and Voltammetric Characterization

Author(s) -

Oana Corduneanu,

AnaMaria ChiorceaPaquim,

Victor C. Diculescu,

Sónia M. Fiuza,

M. Paula M. Marques,

Ana Maria OliveiraBrett

Publication year - 2010

Publication title -

analytical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.117

H-Index - 332

eISSN - 1520-6882

pISSN - 0003-2700

DOI - 10.1021/ac902127d

Subject(s) - chemistry , highly oriented pyrolytic graphite , guanine , palladium , spermine , dna , polyamine , cyclic voltammetry , voltammetry , chelation , crystallography , pyrolytic carbon , electrochemistry , inorganic chemistry , electrode , organic chemistry , graphite , biochemistry , pyrolysis , nucleotide , gene , enzyme , catalysis

The interaction of double-stranded DNA with two polynuclear Pd(II) chelates with the biogenic polyamines spermidine (Spd) and spermine (Spm), Pd(II)-Spd and Pd(II)-Spm, as well as with the free ligands Spd and Spm, was studied using atomic force microscopy (AFM) at a highly oriented pyrolytic graphite (HOPG) surface, voltammetry at a glassy carbon (GC) electrode, and gel electrophoresis. The AFM and voltammetric results showed that the interaction of Spd and Spm with DNA occurred even for a low concentration of polyamines and caused no oxidative damage to DNA. The Pd(II)-Spd and Pd(II)-Spm complexes were found to induce greater morphological changes in the dsDNA conformation, when compared with their ligands. The interaction was specific, inducing distortion and local denaturation of the B-DNA structure with release of some guanine bases. The DNA strands partially opened give rise to palladium intra- and interstrand cross-links, leading to the formation of DNA adducts and aggregates, particularly in the case of the Pd(II)-Spd complex.

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