Droplet-Based Microfluidic Platform for High-Throughput, Multi-Parameter Screening of Photosensitizer Activity | Zendy

Soongwon Cho | Zendy; DongKu Kang | Zendy; Steven Sim | Zendy; Florian Geier | Zendy; Jinyoung Kim | Zendy; Xize Niu | Zendy; Joshua B. Edel | Zendy; SooIk Chang | Zendy; Robert C. R. Wootton | Zendy; Katherine S. Elvira | Zendy; Andrew J. deMello | Zendy

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Droplet-Based Microfluidic Platform for High-Throughput, Multi-Parameter Screening of Photosensitizer Activity

Author(s) -

Soongwon Cho,

DongKu Kang,

Steven Sim,

Florian Geier,

Jinyoung Kim,

Xize Niu,

Joshua B. Edel,

SooIk Chang,

Robert C. R. Wootton,

Katherine S. Elvira,

Andrew J. deMello

Publication year - 2013

Publication title -

analytical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.117

H-Index - 332

eISSN - 1520-6882

pISSN - 0003-2700

DOI - 10.1021/ac4022067

Subject(s) - photosensitizer , chemistry , viability assay , microfluidics , photodynamic therapy , toxicity , high throughput screening , nanotechnology , biophysics , cell , photochemistry , materials science , biochemistry , organic chemistry , biology

We present a fully integrated droplet-based microfluidic platform for the high-throughput assessment of photodynamic therapy photosensitizer (PDT) efficacy on Escherichia coli. The described platform is able to controllably encapsulate cells and photosensitizer within pL-volume droplets, incubate the droplets over the course of several days, add predetermined concentrations of viability assay agents, expose droplets to varying doses of electromagnetic radiation, and detect both live and dead cells online to score cell viability. The viability of cells after encapsulation and incubation is assessed in a direct fashion, and the viability scoring method is compared to model live/dead systems for calibration. Final results are validated against conventional colony forming unit assays. In addition, we show that the platform can be used to perform concurrent measurements of light and dark toxicity of the PDT agents and that the platform allows simultaneous measurement of experimental parameters that include dark toxicity, photosensitizer concentration, light dose, and oxygenation levels for the development and testing of PDT agents.

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