Reversible Cation Response with a Protein-Modified Nanopipette | Zendy

Boaz Vilozny | Zendy; Paolo Actis | Zendy; R Seger | Zendy; Queralt Vallmajó-Martín | Zendy; Nader Pourmand | Zendy

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Reversible Cation Response with a Protein-Modified Nanopipette

Author(s) -

Boaz Vilozny,

Paolo Actis,

R Seger,

Queralt Vallmajó-Martín,

Nader Pourmand

Publication year - 2011

Publication title -

analytical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.117

H-Index - 332

eISSN - 1520-6882

pISSN - 0003-2700

DOI - 10.1021/ac201322v

Subject(s) - chemistry , nanopore , analyte , calmodulin , calcium , buffer (optical fiber) , biophysics , ion , nanotechnology , combinatorial chemistry , chromatography , analytical chemistry (journal) , organic chemistry , telecommunications , materials science , computer science , biology

The calcium ion response of a quartz nanopipette was enhanced by immobilization of calmodulin to the nanopore surface. Binding to the analyte is rapidly reversible in neutral buffer and requires no change in media or conditions to regenerate the receptor. The signal remained reproducible over numerous measurements. The modified nanopipette was used to measure binding affinity to calcium ions, with a K(d) of 6.3 ± 0.8 × 10(-5) M. This affinity is in good agreement with reported values of the solution-state protein. The behavior of such reversible nanopore-based sensors can be used to study proteins in a confined environment and may lead to new devices for continuous monitoring.

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