Hemoglobin Variant Analysis via Direct Surface Sampling of Dried Blood Spots Coupled with High-Resolution Mass Spectrometry | Zendy

Rebecca L. Edwards | Zendy; Andrew J. Creese | Zendy; Mark Baumert | Zendy; Paul Griffiths | Zendy; Josephine Bunch | Zendy; Helen J. Cooper | Zendy

Open Access

Hemoglobin Variant Analysis via Direct Surface Sampling of Dried Blood Spots Coupled with High-Resolution Mass Spectrometry

Author(s) -

Rebecca L. Edwards,

Andrew J. Creese,

Mark Baumert,

Paul Griffiths,

Josephine Bunch,

Helen J. Cooper

Publication year - 2011

Publication title -

analytical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.117

H-Index - 332

eISSN - 1520-6882

pISSN - 0003-2700

DOI - 10.1021/ac1030804

Subject(s) - dried blood spot , chemistry , dried blood , spots , chromatography , hemoglobin , mass spectrometry , resolution (logic) , newborn screening , whole blood , blood sampling , hemoglobin variants , high resolution , electrospray , analytical chemistry (journal) , biochemistry , medicine , remote sensing , artificial intelligence , computer science , geology

Hemoglobinopathies are the most common inherited disorders. Newborn blood screening for clinically significant hemoglobin variants, including sickle (HbS), HbC, and HbD, has been adopted in many countries as it is widely acknowledged that early detection improves the outcome. We present a method for determination of Hb variants by direct surface sampling of dried blood spots by use of an Advion Triversa Nanomate automated electrospray system coupled to a high-resolution mass spectrometer. The method involves no sample preparation. It is possible to unambiguously identify homozygous and heterozygous HbS, HbC, and HbD variants in <10 min without the need for additional confirmation. The method allows for repeated analysis of a single blood spot over a prolonged time period and is tolerant of blood spot storage conditions.

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