Open AccessParallel Nanoliter Microfluidic Analysis System
Author(s) -
Per Andersson,
Gérald Jesson,
Gunnar Kylberg,
Gunnar Ekstrand,
Gunnar Thorsén
Publication year - 2007
Publication title -
analytical chemistry
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 2.117
H-Index - 332
eISSN - 1520-6882
pISSN - 0003-2700
DOI - 10.1021/ac061692y
Subject(s) - chemistry , channel (broadcasting) , microfluidics , fluidics , volume (thermodynamics) , capillary action , biological system , flow (mathematics) , analytical chemistry (journal) , mechanics , nanotechnology , chromatography , computer science , thermodynamics , physics , electrical engineering , materials science , engineering , biology , computer network
A parallel nanoliter microfluidic analysis system based on capillary action, centrifugal force, and hydrophobic barriers is described. The precision of 112 parallel volume definition operations is determined to 0.75% CV at 200 nL using the individual sample introduction structure. For 20 nL, the actual measurement error is the dominating factor, with a combined error of 1.9% CV. Individual dispensing as well as dispensing through a common distribution channel is described. The volume definition precision for the common distribution channel is 1.6% CV for 200 nL. Unlike the dominating forces in microliter-sized channel systems, we describe hysteresis effects as exerting a major influence, which needs to be considered in order to control the operation and design of discrete nanoliter fluidics. Hydrophobic patches at the corners of the rectangular channel control corner-enhanced wicking. Excellent flow control of 1 and 2 nL/s is achieved using a predefined spin program.
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