Investigation of the Chromatographic Process via Pulsed-Gradient Spin−Echo Nuclear Magnetic Resonance. Role of the Solvent Composition in Partitioning Chromatography | Zendy

Guilhem Pagès | Zendy; Corinne Delaurent | Zendy; Stéfano Caldarelli | Zendy

Open Access

Investigation of the Chromatographic Process via Pulsed-Gradient Spin−Echo Nuclear Magnetic Resonance. Role of the Solvent Composition in Partitioning Chromatography

Author(s) -

Guilhem Pagès,

Corinne Delaurent,

Stéfano Caldarelli

Publication year - 2005

Publication title -

analytical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.117

H-Index - 332

eISSN - 1520-6882

pISSN - 0003-2700

DOI - 10.1021/ac051454n

Subject(s) - chemistry , analytical chemistry (journal) , analyte , chromatography , magic angle spinning , spin echo , diffusion , solvent , phase (matter) , acetonitrile , nuclear magnetic resonance , nuclear magnetic resonance spectroscopy , thermodynamics , magnetic resonance imaging , organic chemistry , medicine , physics , radiology

The diffusional properties of molecules in solution vary dramatically upon addition of a solid chromatographic phase. This effect can be monitored via pulsed-gradient spin-echo NMR used in conjunction with moderately fast rotation of the sample (high-resolution magic angle spinning) to produce exploitable spectra. The molecular diffusion coefficients observed in this condition are averages reflecting the equilibrium population distribution among the different phases. It is thus possible to use this information for investigating a crucial step of reversed-phase chromatography, namely, the partitioning of the analyte between different phases. In this work, we describe the evolution of the apparent diffusion coefficient of typical solutes for water/acetonitrile solvent mixtures of varying proportions.

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