A Low-Makeup Beveled Tip Capillary Electrophoresis /Electrospray Ionization Mass Spectrometry Interface for Micellar Electrokinetic Chromatography and Nonvolatile Buffer Capillary Electrophoresis | Zendy

MeiChun Tseng | Zendy; YetRan Chen | Zendy; GuorRong Her | Zendy

Open Access

A Low-Makeup Beveled Tip Capillary Electrophoresis /Electrospray Ionization Mass Spectrometry Interface for Micellar Electrokinetic Chromatography and Nonvolatile Buffer Capillary Electrophoresis

Author(s) -

MeiChun Tseng,

YetRan Chen,

GuorRong Her

Publication year - 2004

Publication title -

analytical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.117

H-Index - 332

eISSN - 1520-6882

pISSN - 0003-2700

DOI - 10.1021/ac049330i

Subject(s) - chemistry , micellar electrokinetic chromatography , capillary electrophoresis , chromatography , electrokinetic phenomena , capillary electrophoresis–mass spectrometry , mass spectrometry , electrospray , electrospray ionization , electrophoresis , analytical chemistry (journal) , capillary action , capillary electrochromatography , buffer (optical fiber) , materials science , composite material , telecommunications , computer science

A robust interface has been developed for interfacing micellar electrokinetic chromatography (MEKC) and nonvolatile buffer capillary electrophoresis (CE) to electrospray ionization mass spectrometry (ESI-MS). The interface consists of two parallel capillaries for separation (50 microm i.d. x 155 microm o.d.) and makeup (50 microm i.d. x 155 microm o.d.) housed within a larger capillary (530 microm i.d. x 690 microm o.d.). The capillaries terminate in a single tapered tip having a beveled edge. The use of a tapered beveled edge results in a greater tip orifice diameter (75 microm) than in a previous design from our laboratory (25 microm) that used a flat tip. While maintaining a similar optimum flow rate and consequently similar sample dilution, a 75-microm beveled emitter is more rugged than a 25-microm flat tip. Furthermore, the incorporation of a sheath liquid capillary allows the compositions of the final spray solution to be controlled. The application of this novel CE/ESI-MS interface was demonstrated for MEKC using mixtures of triazines (positive ion mode) and phenols (negative ion mode). The ability to perform CE/ESI-MS using a nonvolatile buffer was demonstrated by the analysis of gangliosides with a buffer consisting of 40 mM borate and 20 mM alpha-cyclodextrin.

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