Multistep Microreactions with Proteins Using Electrocapture Technology | Zendy

Juan AstorgaWells | Zendy; Tomas Bergman | Zendy; Hans Jörnvall | Zendy

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Multistep Microreactions with Proteins Using Electrocapture Technology

Author(s) -

Juan AstorgaWells,

Tomas Bergman,

Hans Jörnvall

Publication year - 2004

Publication title -

analytical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.117

H-Index - 332

eISSN - 1520-6882

pISSN - 0003-2700

DOI - 10.1021/ac0354342

Subject(s) - chemistry , reagent , trypsin , mass spectrometry , chromatography , electric field , digestion (alchemy) , alkylation , analytical chemistry (journal) , enzyme , biochemistry , organic chemistry , physics , quantum mechanics , catalysis

A method to perform multistep reactions by means of electroimmobilization of a target molecule in a microflow stream is presented. A target protein is captured by the opposing effects between the hydrodynamic and electric forces, after which another medium is injected into the system. The second medium carries enzymes or other reagents, which are brought into contact with the target protein and react. The immobilization is reversed by disconnecting the electric field, upon which products are collected at the outlet of the device for analysis. On-line reduction, alkylation, and trypsin digestion of proteins is demonstrated and was monitored by MALDI mass spectrometry.

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