Open AccessHeterologous expression of Zygosaccharomyces rouxii glycerol 3‐phosphate dehydrogenase gene ( ZrGPD1 ) and glycerol dehydrogenase gene ( ZrGCY1 ) in Saccharomyces cerevisiae
Author(s) -
Watanabe Yasuo,
Tsuchimoto Syoko,
Tamai Youichi
Publication year - 2004
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1016/s1567-1356(03)00210-1
Subject(s) - glycerol , biology , glycerol 3 phosphate dehydrogenase , dehydrogenase , heterologous expression , biochemistry , heterologous , saccharomyces cerevisiae , gene , gene expression , open reading frame , microbiology and biotechnology , enzyme , recombinant dna , peptide sequence
We examined the effects of heterologous expression of the open reading frames (ORF) of two genes on salt tolerance and glycerol production in a Saccharomyces cerevisiae strain deficient in glycerol synthesis ( gpd1 Δ gpd2 Δ). When the ORF of the Zygosaccharomyces rouxii glycerol 3‐phosphate dehydrogenase gene ( ZrGPD1 ) was expressed under the control of the GAL10 promoter, salt tolerance and glycerol production increased; when the ORF of the glycerol dehydrogenase gene ( ZrGCY1 ) was expressed under the control of the GAL1 promoter, no such changes were observed. Zrgcy1p had a weak effect on glycerol production. These results suggest that Zrgpd1p is the primary enzyme involved in Z. rouxii glycerol production, following a mechanism similar to that of S. cerevisiae (Gpd1p). When the ORFs of the S. cerevisiae glycerol 3‐phosphatase gene ( GPP2 ) and ZrGPD1 were simultaneously expressed, glycerol production increased, compared with that in yeast expressing only ZrGPD1 .