Integration of heterologous genes in several yeast species using vectors containing a Hansenula polymorpha ‐derived rDNA‐targeting element

A method that has been successfully used to generate recombinant Hansenula polymorpha strains by transformation with rDNA‐targeting vectors was applied in the present study to a range of alternative yeast hosts, using vectors with an H. polymorpha ‐derived integration sequence. The dimorphic yeast Arxula adeninivorans , which is currently being assessed for heterologous gene expression, was the main focus of the study. As in H. polymorpha , it was possible to co‐integrate more than a single plasmid carrying an expressible gene. Additionally, the vectors were examined in two further species, Pichia stipitis and Saccharomyces cerevisiae . Based on these results the design of a ‘universal’ fungal vector appears to be feasible.