Open AccessComparative analyses of Saccharomyces cerevisiae RNAs using Agilent RNA 6000 Nano Assay and agarose gel electrophoresis
Author(s) -
Řičicová Markéta,
Palková Zdena
Publication year - 2003
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1016/s1567-1356(03)00145-4
Subject(s) - rna , biology , agarose gel electrophoresis , microbiology and biotechnology , ethidium bromide , agarose , rnase p , gel electrophoresis , nuclease protection assay , complementary dna , dna , rna extraction , biochemistry , non coding rna , gene
Precise quantification and quality characterisation of isolated RNAs are prerequisites for their further exploitation in genome‐wide microarrays, Northern blots, cDNA library preparation and others. Our data indicate that RNA analyses using Agilent RNA Nano Assay exhibit several advantages when compared with those performed on ethidium bromide‐stained agarose gel electrophoresis or on a spectrophotometer. The RNA Nano Assay makes it possible to estimate RNA concentrations in the range from 1000 ng μl −1 to 17 ng μl −1 . The presence of impurities including traces of DNA within RNA samples does not influence the concentration measurements. Like agarose gel electrophoresis, RNA Nano Assay allows to analyse RNAs dissolved in formamide and therefore protected against RNase action. Moreover, it allows a clearer distinction of partially degraded samples. The limitation of RNA Nano Assay is the impossibility to detect and to analyse double‐stranded RNAs.