Open AccessThe efficiency of functional mitochondrial replacement in Saccharomyces species has directional character
Author(s) -
Sulo Pavol,
Špírek Mário,
Šoltésová Andrea,
Marii Gaelle,
Piškur Jure
Publication year - 2003
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1016/s1567-1356(03)00109-0
Subject(s) - biology , mitochondrion , saccharomyces cerevisiae , oxidative phosphorylation , mitochondrial dna , nuclear dna , genome , mutant , nuclear gene , genetics , microbiology and biotechnology , biochemistry , gene
Optimal interactions among nuclear and mitochondria‐coded proteins are required to assemble functional complexes of mitochondrial oxidative phosphorylation. The communication between the nuclear and mitochondrial genomes has been studied by transplacement of mitochondria from related species into mutants devoid of mitochondrial DNA (ρ°). Recently we have reported that the mitochondria transferred from Saccharomyces paradoxus restored partially the respiration in Saccharomyces cerevisiae ρ° mutants. Here we present evidence that the S. cerevisiae mitochondria completely salvage from respiration deficiency, not only in conspecific isolates but also in S. paradoxus . The respiratory capacity in less‐related species can be recovered exclusively in the presence of S. cerevisiae chromosomes. The efficiency of the re‐established oxidative phosphorylation did not rely on the presence of introns in the S. cerevisiae mitochondrial DNA. Our results suggest that, apart from evolutionary distance, the direction of mitochondrial replacement could play a significant role in installing the complete (wild‐type‐like) interaction between mitochondria and nuclei from different species.