Open AccessRole of nitric oxide in the response of Saccharomyces cerevisiae cells to heat shock and high hydrostatic pressure
Author(s) -
Domitrovic Tatiana,
Palhano Fernando L,
BarjaFidalgo Christina,
DeFreitas Martha,
Orlando Marcos T.D.,
Fernandes Patricia M.B.
Publication year - 2003
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1016/s1567-1356(03)00039-4
Subject(s) - intracellular , saccharomyces cerevisiae , hydrostatic pressure , nitric oxide , biology , shock (circulatory) , microbiology and biotechnology , nitric oxide synthase , second messenger system , biophysics , heat shock protein , biochemistry , yeast , gene , medicine , physics , endocrinology , thermodynamics
Nitric oxide (NO) is a simple and unique molecule that has diverse functions in organisms, including intracellular and intercellular messenger. The influence of NO on cell growth of Saccharomyces cerevisiae and as a signal molecule in stress response was evaluated. Respiring cells were more sensitive to an increase in intracellular NO concentration than fermentatively growing cells. Low levels of NO demonstrated a cytoprotective effect during stress from heat‐shock or high hydrostatic pressure. Induction of NO synthase was isoform‐specific and dependent on the metabolic state of the cells and the stress response pathway. These results support the hypothesis that an increase in intracellular NO concentration leads to stress protection.