Open AccessGenome comparisons in the genus Dipodascus de Lagerheim
Author(s) -
Smith Maudy Th,
Poot G.A.
Publication year - 2003
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1016/s1567-1356(03)00013-8
Subject(s) - biology , geotrichum , genus , genome , taxon , dna , nuclear dna , genomic dna , similarity (geometry) , zoology , genome size , genetics , evolutionary biology , gene , botany , mitochondrial dna , artificial intelligence , computer science , image (mathematics)
Phenotypic characteristics of physiology and morphology of 71 strains belonging to the genus Dipodascus de Lagerheim were examined. The GC contents of genomic DNAs of 46 strains were calculated from the thermal denaturation curves using the spectrophotometric method. The first derivatives of the melting curves revealed that the DNAs of these strains are heterogeneous; four categories could be recognized. However, DNA similarity values calculated by using DNA–DNA reassociation kinetics showed that each category could be subdivided further. Two categories were separated into four subgroups each; the other two yielded five subgroups each. Strains belonging to the same subgroup exhibited high levels of DNA similarity ranging from 82 to 100%. The 18 subgroups represented 13 currently accepted Dipodascus species and five anamorphic Geotrichum species, four representing novel taxa. A phenotypic key to distinguish the taxa of Dipodascus , Galactomyces and Geotrichum is presented.