Open AccessATP leakage from yeast cells treated by extracellular glycolipids of Pseudozyma fusiformata
Author(s) -
Kulakovskaya Tatiana V,
Kulakovskaya Ekaterina V,
Golubev Wladyslav I
Publication year - 2003
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1016/s1567-1356(02)00202-7
Subject(s) - biology , yeast , glycolipid , extracellular , leakage (economics) , biochemistry , microbiology and biotechnology , macroeconomics , economics
The ustilaginaceous yeast Pseudozyma fusiformata secreted glycolipids which were lethal to many yeasts and fungi more active at pH of about 4.0, and in the temperature range of 20–30°C. Purified glycolipids enhanced non‐specific permeability of the cytoplasmic membrane in sensitive cells, which resulted in ATP leakage and susceptibility of the cells to staining with bromocresol purple. Cells of Saccharomyces cerevisiae lost the ability to acidify the medium. Basidiomycetous yeasts were more sensitive to the glycolipids than ascomycetous ones. The minimal effective glycolipid concentration was 0.13 and 0.26 mg ml −1 for Cryptococcus terreus and Filobasidiella neoformans , while for Candida albicans and Saccharomyces cerevisiae it was 1.0 and 1.6 mg ml −1 .