Open AccessMolecular genetic identification of Saccharomyces sensu stricto strains from African sorghum beer
Author(s) -
Naumova Elena S,
Korshunova Irina V,
Jespersen Lene,
Naumov Gennadi I
Publication year - 2003
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1016/s1567-1356(02)00191-5
Subject(s) - biology , haeiii , restriction fragment length polymorphism , genetics , internal transcribed spacer , hpaii , ribosomal dna , polymerase chain reaction , microsatellite , spacer dna , ribosomal rna , gene , phylogenetic tree , allele , dna methylation , gene expression
Genetic relationships of 24 phenotypically different strains isolated from sorghum beer in West Africa and the type cultures of the Saccharomyces sensu stricto species were investigated by universally primed polymerase chain reaction (PCR) analysis, microsatellite fingerprinting and PCR‐restriction fragment length polymorphism (RFLP) of the ribosomal internal transcribed spacers. The results demonstrate that internal transcribed spacer (ITS) PCR‐RFLP analysis with the endonucleases Hae III, Hpa II, Scr FI and Taq I is useful for discriminating S. cerevisiae , S. kudriavzevii , S. mikatae from one another and from the S. bayanus / S. pastorianus and S. cariocanus / S. paradoxus pairs. The sorghum beer strains exhibited the same restriction patterns as the type culture of S. cerevisiae CBS 1171. PCR profiles generated with the microsatellite primer (GTG) 5 and the universal primer N21 were almost identical for all isolates and strain CBS 1171. Despite phenotypic peculiarities, the strains involved in sorghum beer production in Ghana and Burkina Faso belong to S. cerevisiae . However, based on sequencing of the rDNA ITS1 region and Southern hybridisation analysis, these strains represent a divergent population of S. cerevisiae .