Open AccessPhysiological behaviour of Hanseniaspora guilliermondii in aerobic glucose‐limited continuous cultures
Author(s) -
Albergaria Helena,
Torrão Ana R,
Hogg Timothy,
Gírio Francisco M
Publication year - 2003
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1016/s1567-1356(02)00187-3
Subject(s) - chemostat , dilution , biology , glycerol , yield (engineering) , food science , biochemistry , ethanol , biomass (ecology) , yeast , carbon dioxide , ecology , bacteria , physics , genetics , materials science , metallurgy , thermodynamics
The physiology of Hanseniaspora guilliermondii was studied under aerobic glucose‐limited conditions using the accelerostat procedure (continuous acceleration of dilution rate) and classical chemostat cultures. By both cultivation techniques this yeast was found to be Crabtree‐positive. Up to a dilution rate of 0.25 h −1 , glucose was completely metabolised into biomass, glycerol and carbon dioxide. Above this value, an increase in the dilution rate was accompanied by the production of other metabolites like ethanol, acetic and malic acids. Biomass yield during the purely oxidative growth was 0.49 g g −1 and decreased to 0.26 g g −1 for D =0.34 h −1 . A maximal specific ethanol production rate of 1.36 mmol g −1 h −1 and a maximal ethanol yield of 0.05 g g −1 were achieved at D =0.34 h −1 .