Open AccessESI-FTICR mass spectrometry employing Data-dependent external ion selection and accumulation
Author(s) -
Richard Harkewicz,
Mikhail E. Belov,
Gordon A. Anderson,
Ljiljana PašaTolić,
Christophe Masselon,
David C. Prior,
Harold R. Udseth,
Richard Smith
Publication year - 2002
Publication title -
journal of the american society for mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.961
H-Index - 127
eISSN - 1879-1123
pISSN - 1044-0305
DOI - 10.1016/s1044-0305(01)00343-9
Subject(s) - chemistry , fourier transform ion cyclotron resonance , mass spectrometry , ion , quadrupole ion trap , analytical chemistry (journal) , quadrupole , selection (genetic algorithm) , ion trap , top down proteomics , tandem mass spectrometry , chromatography , atomic physics , selected reaction monitoring , physics , computer science , organic chemistry , artificial intelligence
Data-dependent external m/z selection and accumulation of ions is demonstrated in use with ESI-FTICR instrumentation, with two different methods for ion selection being explored. One method uses RF/DC quadrupole filtering and is described in use with an 11.5 tesla (T) FTICR instrument, while the second method employs RF-only resonance dipolar excitation selection and is described in use with a 3.5 T FTICR instrument. In both methods ions are data-dependently selected on the fly in a linear quadrupole ion guide, then accumulated in a second linear RF-only quadrupole trap that immediately follows. A major benefit of ion preselection prior to external accumulation is the enhancement of ion populations for low-level species. This development is expected to expand the dynamic range and sensitivity of FTICR for applications including analysis of complex polypeptide mixtures (e.g., proteomics).