Open AccessIdentification of genomic groups in the genus Stenotrophomonas using gyrB RFLP analysis
Author(s) -
Coenye Tom,
Vanlaere Elke,
LiPuma John J,
Vandamme Peter
Publication year - 2004
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1016/s0928-8244(03)00307-9
Subject(s) - stenotrophomonas maltophilia , biology , restriction fragment length polymorphism , stenotrophomonas , genomic dna , microbiology and biotechnology , polymerase chain reaction , genetics , gene , pseudomonas aeruginosa , bacteria , 16s ribosomal rna
Stenotrophomonas maltophilia isolates have been recovered from various clinical samples, including the respiratory tract of cystic fibrosis (CF) patients, but this organism is also widespread in nature. Previously it has been shown that there is a considerable genomic diversity within S. maltophilia . The aims of our study were to determine the taxonomic resolution of restriction fragment length polymorphism (RFLP) analysis of the polymerase chain reaction‐amplified gyrB gene for the genus Stenotrophomonas . Subsequently, we wanted to use this technique to screen a set of S. maltophilia isolates (with emphasis on a specific subset, isolates recovered from CF patients), to assess the genomic diversity within this group. In this study we investigated 191 Stenotrophomonas sp. isolates (including 40 isolates recovered from CF patients) by means of gyrB RFLP. The taxonomic resolution of gyrB RFLP, and hence its potential as an identification tool, was confirmed by comparison with results from published and novel DNA–DNA hybridisation experiments. Our data also indicate that the majority of CF isolates grouped in two clusters. This may indicate that isolates from specific genomic groups have an increased potential for colonisation of the respiratory tract of CF patients.