Open AccessDevelopment and validation of a new in vitro assay for selection of probiotic bacteria that express immune‐stimulating properties in chickens in vivo
Author(s) -
Koenen Marjorie E,
Hulst Remy,
Leering Mariska,
Jeurissen Suzan H.M,
Boersma Wim J.A
Publication year - 2004
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1016/s0928-8244(03)00306-7
Subject(s) - in vivo , bacteria , biology , probiotic , lactic acid , immune system , in vitro , microbiology and biotechnology , in vitro toxicology , microorganism , immunology , biochemistry , genetics
Oral administration of immunoprobiotic bacteria may support animal health. Species specificity of such microorganisms requires appropriate selection. An in vitro assay for the selection of immunoprobiotic lactic acid bacteria was developed in chicken. The assay allowed testing of large numbers of individual strains. Immune stimulation in vitro correlated well with the in vivo situation in two experiments and no false negative results occurred. Therefore this assay is an appropriate selection tool for immunomodulating properties of lactic acid bacteria in chicken.