Open AccessIdentification of pathogenic dematiaceous fungi and related taxa based on large subunit ribosomal DNA D1/D2 domain sequence analysis
Author(s) -
Abliz Paride,
Fukushima Kazutaka,
Takizawa Kayoko,
Nishimura Kazuko
Publication year - 2004
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1016/s0928-8244(03)00275-x
Subject(s) - biology , ribosomal dna , microbiology and biotechnology , phylogenetic tree , ribosomal rna , genetics , gene
The nucleotide sequences of the D1/D2 domains of large subunit (26S) ribosomal DNA for 76 strains of 46 species of pathogenic dematiaceous fungi and related taxa were determined. Intra‐species sequence diversity of medically important dematiaceous fungi including Phialophora verrucosa , Fonsecaea pedrosoi , Fonsecaea compacta , Cladophialophora carrionii , Cladophialophora bantiana , Exophiala dermatitidis , Exophiala jeanselmei , Exophiala spinifera , Exophiala moniliae , and Hortaea werneckii were extremely small; as few as 0 changes were detected in C. bantiana , Fonsecaea and Exophiala species, 1 bp in C. carrionii and H. werneckii , and 2 bp in P. verrucosa . Inter‐species nucleotide diversity between most species was higher. These data suggested that the D1/D2 domain is sufficiently variable for identification of pathogenic dematiaceous fungi and relevant species. The phylogenetic trees constructed from the sequence data revealed that most human pathogenic species formed a single cluster and that Cladosporium and Phialophora species were distributed polyphyletically into several clusters.