Open AccessSerum high‐density lipoprotein (HDL) inhibits in vitro enterohemolysin (EHly) activity produced by enteropathogenic Escherichia coli
Author(s) -
Figueirêdo Patricia M.S,
Catani Cleide F,
Yano Tomomasa
Publication year - 2003
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1016/s0928-8244(03)00125-1
Subject(s) - chylomicron , biology , in vitro , escherichia coli , hemolysin , lipoprotein , microbiology and biotechnology , biochemistry , cholesterol , high density lipoprotein , very low density lipoprotein , virulence , gene
Enterohemolysin (EHly) produced by Escherichia coli shows hemolytic activity towards washed erythrocytes from different animal species on blood agar plates. It has been shown recently that EHly activity is inhibited by normal mammalian serum and by cholesterol in vitro. Plasma lipoproteins can interact with bacterial toxins, such as endotoxin, to reduce their toxicity. In this work, we examine the ability of human purified chylomicrons, very low‐density lipoproteins, intermediate‐density, low‐density and high‐density lipoproteins, to inhibit the hemolytic activity of EHly. Our results show that these lipoproteins are hemolysin inactivators, and that high‐density lipoprotein is the most potent inhibitor of enterohemolytic activity.