Open AccessThe acute phase protein serum amyloid A primes neutrophils
Author(s) -
Hatanaka Elaine,
Pereira Ribeiro Fernanda,
Campa Ana
Publication year - 2003
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1016/s0928-8244(03)00112-3
Subject(s) - zymosan , respiratory burst , opsonin , acute phase protein , reactive oxygen species , serum amyloid a , incubation , phorbol , biology , receptor , oxidative stress , phagocytosis , inflammation , neutrophile , immunology , endocrinology , biochemistry , protein kinase c , in vitro , signal transduction
We studied here the effect of the acute phase protein serum amyloid A (SAA) on the oxidative burst of neutrophils. Incubation of neutrophils with SAA increased the rate of oxygen uptake and the production of reactive oxygen species of neutrophils activated with opsonized zymosan (OZ). The increment in the neutrophil oxidative burst was dependent on SAA concentration in the range of 3–33 μg protein ml −1 and was observed only in the presence of a relatively low amount of OZ (1×10 6 particles ml −1 ). SAA did not affect oxygen consumption and reactive oxygen production triggered by other stimuli, such as f‐Met‐Leu‐Phe, phorbol myristate acetate or non‐opsonized zymosan. Our finding points to a priming effect of SAA probably associated with mobilization of receptors for opsonized particles and strengthens the role of SAA as an effector of neutrophil functions in inflammation.