Open AccessEndotoxic and immunobiological activities of a chemically synthesized lipid A of Helicobacter pylori strain 206–1
Author(s) -
Ogawa Tomohiko,
Asai Yasuyuki,
Sakai Yasuhiro,
Oikawa Masato,
Fukase Koichi,
Suda Yasuo,
Kusumoto Shoichi,
Tamura Toshihide
Publication year - 2003
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1016/s0928-8244(03)00093-2
Subject(s) - lipid a , lipopolysaccharide , biology , peripheral blood mononuclear cell , helicobacter pylori , monoclonal antibody , tlr4 , cytokine , strain (injury) , microbiology and biotechnology , tumor necrosis factor alpha , lipid metabolism , spleen , interleukin 2 , antibody , receptor , biochemistry , immunology , in vitro , genetics , anatomy
A synthetic lipid A of Helicobacter pylori strain 206‐1 (compound HP206–1), which is similar to its natural lipid A, exhibited no or very low endotoxic activities as compared to Escherichia coli ‐type synthetic lipid A (compound 506). Furthermore, compound HP206‐1 as well as its natural lipid A demonstrated no or very low mitogenic responses in murine spleen cell. On the other hand, compound HP206‐1 showed a weaker but significant production of interleukin‐8 in a gastric cancer cell line, MKN‐1, in comparison with compound 506. Furthermore, compound HP206‐1 exhibited induction of tumor necrosis factor‐α production in human peripheral blood mononuclear cells and the cytokine production was clearly inhibited by mouse anti‐human Toll‐like receptor (TLR) 4 monoclonal antibody HTA125. Our findings indicate that the chemically synthesized lipid A, mimicking the natural lipid A portion of lipopolysaccharide from H. pylori strain 206‐1, has a low endotoxic potency and immunobiological activities, and is recognized by TLR4.