Open AccessDifferences in immunogenicity and protection in mice and guinea pigs following intranasal immunization with Helicobacter pylori outer membrane antigens 1
Author(s) -
Keenan Jacqueline I,
Rijpkema Sjoerd G,
Durrani Zarmina,
Roake Justin A
Publication year - 2003
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1016/s0928-8244(03)00091-9
Subject(s) - immunogenicity , bacterial outer membrane , biology , antigen , immunization , immune system , helicobacter pylori , nasal administration , adjuvant , cholera toxin , immunology , microbiology and biotechnology , antibody , vaccination , virology , biochemistry , genetics , escherichia coli , gene
Mice and guinea pigs were intranasally immunized with either recombinant lipoprotein 20 or Helicobacter pylori outer membrane vesicles (OMV). Cholera toxin was used as mucosal adjuvant. In mice, both vaccines elicited systemic and local IgG responses, which correlated with significantly lower levels of H. pylori colonization. In contrast, only OMV proved immunogenic in guinea pigs, with the development of both systemic and local immune responses. These antibodies did not, however, correlate with protection in these animals, which suggests that vaccine formulation is as important as choice of antigen in the development of an H. pylori vaccine.