Open AccessImmune responses dependent on antigen location in recombinant attenuated Salmonella typhimurium vaccines following oral immunization
Author(s) -
Kang Ho Young,
Curtiss Roy
Publication year - 2003
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1016/s0928-8244(03)00063-4
Subject(s) - immunization , salmonella , biology , immune system , antigen , recombinant dna , virology , microbiology and biotechnology , immunology , vaccination , immunity , attenuated vaccine , bacteria , virulence , genetics , gene
The subcellular location of a recombinant antigen in recombinant attenuated Salmonella vaccines may influence immunogenicity dependent on exposure of the recombinant antigen to cells involved in systemic immune responses. It has been shown that a recombinant attenuated Salmonella vaccine secreting the recombinant Streptococcus pneumoniae PspA (rPspA) antigen specified by pYA3494 induced protective anti‐rPspA‐specific immune responses (Kang et al. (2002) Infect. Immun. 70, 1739–1749). A recombinant plasmid pYA3496 specifying a His 6 ‐tagged rPspA (His 6 ‐rPspA) protein (no apparent signal sequence) caused the rPspA antigen to localize to the cytoplasm of Salmonella . Salmonella vaccines carrying pYA3494 or pYA3496 expressed similar amounts of rPspA. After a single oral immunization in BALB/c mice with 10 9 colony‐forming units (CFU) of the recombinant Salmonella vaccines carrying pYA3494 or pYA3496, IgG antibody responses were stimulated to both rPspA and Salmonella lipopolysaccharide (LPS) antigens. The anti‐rPspA IgG titer induced by Salmonella carrying pYA3494 (1.9×10 7 ) was 10 4 times higher than induced by Salmonella carrying pYA3496 (<2.4×10 3 ).