Open AccessLack of biofilm contribution to bacterial colonisation in an experimental model of foreign body infection by Staphylococcus aureus and Staphylococcus epidermidis
Author(s) -
Francois Patrice,
Tu Quoc Patrick H,
Bisognano Carmelo,
Kelley William L,
Lew Daniel P,
Schrenzel Jacques,
Cramton Sarah E,
Götz Friedrich,
Vaudaux Pierre
Publication year - 2003
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1016/s0928-8244(02)00463-7
Subject(s) - staphylococcus epidermidis , staphylococcus aureus , microbiology and biotechnology , biofilm , biology , micrococcaceae , staphylococcal infections , staphylococcus , bacteria , genetics
The contribution of in vivo biofilm‐forming potential of Staphylococcus aureus and Staphylococcus epidermidis was studied in an experimental model of foreign body infections. Increasing inocula (from 10 2 to 10 7 organisms) of ica ‐positive strains of S. aureus and S. epidermidis and their ica ‐negative isogenic mutants (the ica locus codes for a major polysaccharide component of biofilm) were injected into subcutaneously implanted tissue cages in guinea pigs. Surprisingly, bacterial counts and time‐course of tissue cage infection by ica ‐positive strains of S. aureus or S. epidermidis were equivalent to those of their respective ica ‐negative mutants, in the locally infected fluids and on tissue‐cage‐inserted plastic coverslips.