Open AccessCD8 T cell immunome analysis of Listeria monocytogenes
Author(s) -
Kamm Christian,
Škoberne Mojca,
Geginat Gernot
Publication year - 2003
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1016/s0928-8244(02)00450-9
Subject(s) - elispot , biology , ex vivo , listeria monocytogenes , t cell , cd8 , microbiology and biotechnology , cytotoxic t cell , antigen , listeria , immune system , immunology , in vitro , bacteria , biochemistry , genetics
The identification of T cell epitopes is crucial for the understanding of the host response during infections with pathogenic microorganisms. Generally, the identification of relevant T cell responses is based on the analysis of T cell lines propagated in vitro. We used an ex vivo approach for the analysis of the CD8 T cell response against Listeria monocytogenes that is based upon the fractionation of naturally processed antigenic peptides and subsequent analysis with T cells in an enzyme‐linked immunospot (ELISPOT) assay. Our data indicate that the direct ex vivo ELISPOT analysis of peptides extracted from infected tissues represents a versatile and potent test system for the analysis of the CD8 T cell immunome of microorganisms that furthermore requires neither the knowledge of the microbial genome nor of the specificity of responding T cells.