Open AccessWhich morphological forms of the fungus Aureobasidium pullulans are responsible for pullulan production?
Author(s) -
Campbell Bradley S,
Siddique AbuBaker M,
McDougall Barbara M,
Seviour Robert J
Publication year - 2004
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/s0378-1097(04)00076-x
Subject(s) - pullulan , aureobasidium pullulans , pullulanase , fungus , hypha , chlamydospore , chemistry , biology , microbiology and biotechnology , botany , polysaccharide , biochemistry , starch , fermentation
Attempts were made to clarify the precise location and possible site of production of the α‐glucan pullulan in different morphological forms of the fungus Aureobasidium pullulans . Gold‐conjugated pullulanase was used as the specific probe for this purpose. No cell wall pullulan‐like material was detected by transmission electron microscopy (TEM) in any morphological form of this fungus, although intracellular electron transparent material bound this probe. When silver enhancement of this gold‐conjugated pullulanase probe was used, the data strongly suggested that only swollen cells and chlamydospores, and neither hyphae nor unicellular blastospores, often held responsible for pullulan formation, appeared to produce pullulan‐like material.