Open AccessIdentification and characterization of pltZ , a gene involved in the repression of pyoluteorin biosynthesis in Pseudomonas sp. M18
Author(s) -
Huang Xianqing,
Zhu Donghua,
Ge Yihe,
Hu Hongbo,
Zhang Xuehong,
Xu Yuquan
Publication year - 2004
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/s0378-1097(04)00074-6
Subject(s) - psychological repression , pseudomonas , identification (biology) , biosynthesis , gene , biology , pseudomonadales , pseudomonadaceae , biochemistry , genetics , bacteria , gene expression , botany
A new regulator gene named pltZ , which is located downstream of the plt gene cluster in the genome of Pseudomonas sp. M18, was identified, sequenced and characterized in this report. The deduced amino acid sequence of PltZ shares significant homology with other bacterial regulators in the TetR family. The chromosomal pltZ disruption mutant gave rise to 4.4‐fold enhancement of pyoluteorin biosynthesis but did not exert significant influence on the accumulation of phenazine‐1‐carboxylic acid compared with the wild‐type M18. The negative regulation of pltZ on pyoluteorin biosynthesis was further confirmed by multiplied pltZ gene dosage experiments and pltA ′‐′ lacZ translational fusion analyses.