Open AccessQuantification of toxin‐encoding mRNA from Clostridium botulinum type E in media containing sorbic acid or sodium nitrite by competitive RT‐PCR
Author(s) -
Sharkey Freddie H,
Markos Spiros I,
Haylock Richard W
Publication year - 2004
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/s0378-1097(04)00043-6
Subject(s) - sorbic acid , sodium nitrite , clostridium botulinum , toxin , chemistry , messenger rna , biochemistry , microbiology and biotechnology , nitrite , food science , biology , gene , organic chemistry , nitrate
Competitive reverse transcription polymerase chain reaction (cRT‐PCR) was used to quantify the toxin‐encoding mRNA production of a Clostridium botulinum type E strain in media containing either sorbic acid or sodium nitrite. A 10‐fold reduction in toxin mRNA production and a 25‐fold reduction in the proportion of toxin mRNA to total RNA, was estimated when either 1 mg ml −1 sorbic acid or 100 μg ml −1 sodium nitrite were added to the medium at pH 7.0.