Open AccessColony aggregation and biofilm formation in xylem chemistry‐based media for Xylella fastidiosa
Author(s) -
Leite Breno,
Andersen Peter Craig,
Ishida Maria Lucia
Publication year - 2004
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/s0378-1097(03)00917-0
Subject(s) - xylella fastidiosa , xylem , chemistry , strain (injury) , biofilm , botany , biophysics , bacteria , biology , anatomy , genetics
Two chemically defined media based on xylem fluid chemistry were developed for Xylella fastidiosa . These media were tested and compared to chemically defined media XDM 2 , XDM 4 and XF‐26. New media were evaluated for the Pierce's disease (PD) strain UCLA‐PD. Our media either was similar to the concentration of some amino acids found in the xylem fluid of the PD‐susceptible Vitis vinifera cv. Chardonnay (medium CHARD2) or incorporated the tripeptide glutathione found in xylem fluid composition (medium 3G10‐R). CHARD2 and 3G10‐R are among the simplest chemically defined media available. Xylem fluid chemistry‐based media supported X. fastidiosa growth and especially stimulated aggregation and biofilm formation.