Open AccessCharacterization of six type A strains of Clostridium botulinum that contain type B toxin gene sequences
Author(s) -
Kirma Nameer,
Ferreira Joseph L,
Baumstark Barbara R
Publication year - 2004
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/s0378-1097(03)00911-x
Subject(s) - clostridium botulinum , toxin , neurotoxin , biology , gene , clostridiaceae , open reading frame , microbiology and biotechnology , sequence analysis , polymerase chain reaction , dna , strain (injury) , nucleic acid sequence , genetics , peptide sequence , biochemistry , anatomy
Six Clostridium botulinum isolates exhibiting type A toxicity as measured by the mouse bioassay were found to contain both type A and type B neurotoxin DNA sequences. The six strains were divided into three groups based on the DNA sequence of the type B neurotoxin gene. Members of each group exhibited 100% sequence identity over the 3876 bp type B toxin open reading frame. The type B toxin sequence of all groups differed at more than 60 positions when compared to the BGB control strain.