Open AccessExpression of the landomycin biosynthetic gene cluster in a PKS mutant of Streptomyces fradiae is dependent on the coexpression of a putative transcriptional activator gene
Author(s) -
Mulert Ursula,
Luzhetskyy Andriy,
Hofmann Carsten,
Mayer Almuth,
Bechthold Andreas
Publication year - 2004
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/s0378-1097(03)00861-9
Subject(s) - streptomyces fradiae , gene cluster , mutant , polyketide synthase , streptomyces , gene , biology , cosmid , heterologous expression , heterologous , genetics , glycosyltransferase , activator (genetics) , polyketide , actinomycetales , biosynthesis , recombinant dna , bacteria
The formation of landomycin A or one of its derivatives (5,6‐anhydrolandomycin A) in a heterologous strain has never been achieved. It has now been made possible by the coexpression of a cosmid containing all biosynthetic genes necessary to produce landomycin A together with a pathway‐specific regulatory gene. As host we used a polyketide synthase‐defective mutant strain of Streptomyces fradiae Tü2717 which is not able to produce urdamycin A. Our results indicate that four glycosyltransferases are responsible for the formation of the hexasaccharide side chain of landomycin A.